Vitamin D3 (human) ELISA kit

Vitamin D3 (human) ELISA kitis available at Gentaur for Next week Delivery.

Sensitive, Colorimetric Assay.

Description:

Vitamin D3 (Cholecalciferol) is found in human epidermis and dermis. BioVision’s human Vit. D3 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human Vit. D3 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Vit. D3 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Vit. D3 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 20 – 800 µg/L.

Applications: This ELISA kit is used for quantitative protein detection, establishing normal range, validation of antibody array results

Sample Typ:e • Serum & plasma (EDTA/Citrate) • Urine • Cell culture medium, Tissue homogenates and cell lysates

Alternate Name: Human Vitamin D3 (Cholecalciferol) ELISA kit

Features and Benefits:

• Easy, convenient and time-saving method to assay for human vitamin D3. • Detection Range:200 – 800 ug/L. • This ELISA kit shows no species cross-reactivity.