Prostaglandin E1 (PGE1) ELISA Kit

Prostaglandin E1 (PGE1) ELISA Kitis available at Gentaur for Next week Delivery.

The competitive ELISA Kit for in vitro quantitative determination of PGE1 concentrations in serum, plasma and other biological fluids..

Description:

BioVision's PGE1 ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE1. During the reaction, PGE1 in the sample or standard competes with a fixed amount of PGE1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGE1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase(HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications: Specificity: This kit recognizes PGE1 in samples. No significant cross-reactivity or interference between PGE1 and analogues was observed., Detection Range: 78.13—5000 pg/mL, Repeatability: Coefficient of variation is < 10%., in vitro quantitative determination of PGE1 concentrations in serum, plasma and other biological fluids., Sensitivity: 46.88 pg/mL

Sample Typ:e Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids, Tissue and cell lysates

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