Phospholipase D (PLD) Activity Colorimetric Assay Kit

Phospholipase D (PLD) Activity Colorimetric Assay Kitis available at Gentaur for Next week Delivery.

Highly Sensitive Assay, HTS.

Description:

Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders. BioVision’s Phospholipase D Activity Assay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline from Phosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). This high-throughput adaptable assay kit can detect PLD activity as low as 1.0 mU/ml in a variety of samples.

Applications: • Measurement of PLD activity in various tissues/cells • Analyzing intracellular signaling • Screening of PLD inhibitors

Sample Typ:e • Animal tissues: liver, brain, kidney, etc. • Cell culture: Adherent or suspension cells

Alternate Name: Lecithinase D Activity Colorimetric Assay Kit, Choline Phosphatase Activity Colorimetric Assay Kit, Lipophosphodiesterase II Activity Colorimetric Assay Kit

Features and Benefits:

• Simple, rapid & convenient • The assay can detect phospholipase D (PLD) Activity less than 1 mU/ml in various sample types.