Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric)

Phenylalanine Ammonia-Lyase Activity Assay Kit (Fluorometric)is available at Gentaur for Next week Delivery.

To measure the activity of Phenylalanine Ammonia-Lyase in samples.

Description:

Phenylalanine Ammonia-Lyase (PAL, EC 4.3.1.24) catalyzes the reversible non-oxidative deamination of L-phenylalanine to trans-cinnamic acid and ammonia without any external cofactors. PAL is expressed in plants, yeast and some fungi and is absent in bacteria and animals. Patients suffering from the genetic disorder, phenylketonuria is unable to metabolize phenylalanine that leads to irreversible cognitive impairment. Historically, phenylketonuria has been treated with a phenylalanine-restricted diet taken for the entirety of the patient’s life. Recently, a drug containing PEGylated PAL has been FDA-approved to treat phenylketonuria, limiting the need of a restricted diet. BioVision's Phenylalanine Ammonia-Lyase Activity Assay Kit measures the activity of PAL via a two-step plate-based assay. The products of the PAL enzymatic reaction, generated in the first step, react with the developers resulting in the formation of a fluorescent compound, which is detected at Ex/Em = 410/470 nm. The kit can detect as low as 3 µUnits (3 pmol substrate catalysis per minute) of PAL under assay conditions.

Applications: Measurement of Phenylalanine ammonia-lyase activity in samples

Sample Typ:e Purified protein or recombinant enzyme (must be free of primary amines including ammonium sulfate),Serum

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Features and Benefits:

The assay is simple, sensitive, and can detect as low as 3 µU (3 pmol substrate catalysis/minute) of PAL under assay conditions.,A facile, rapid way to measure Phenylalanine ammonia-lyase activity in samples.