Peroxidase Activity Colorimetric/Fluorometric Assay Kit

Peroxidase Activity Colorimetric/Fluorometric Assay Kitis available at Gentaur for Next week Delivery.

Highly Sensitive Assay, HTS.

Description:

Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues. BioVision’s Peroxidase Assay Kit provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.

Applications:

Sample Typ:e Cell and tissue culture supernatant, urine, serum and plasma, as well as many other biological fluids

Alternate Name:

Features and Benefits:

• Simple procedure • Fast and convenient • The assay is simple, direct, highly sensitive and high-throughput adaptable