BioVision Elisa
N′-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit
- SKU:
- K822-100
Description
N′-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kitis available at Gentaur for Next week Delivery.
The FIRST and ONLY kit on the market for Nicotinamide N-Methyltransferase (NNMT) Inhibitor Screening.
Description:
N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. Biovision’s NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.
Applications: Screening/studying/characterizing potential inhibitors of NNMT
Sample Typ:e
Alternate Name:
Features and Benefits:
• Rapid assay and simple protocol • Reliable test for screening potential inhibitors of NNMT • Includes Inhibitor Control, 1-Methylnicotinamide (MNA)
Additional Information
Size: |
100 assays |
Storage Conditions: |
4°C-80°C |
Shipping Conditions: |
dry ice |
Shelf life: |
12 months |
Detection Method: |
Fluorescence (Ex/Em = 392/482 nm) |
Category: |
NNMT, Nicotinamide N-Methyltransferase |