ApoBrdU DNA Fragmentation Assay Kit

ApoBrdU DNA Fragmentation Assay Kitis available at Gentaur for Next week Delivery.

Detects DNA Fragmentation by FACS or FL.

Description:

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.

Applications:

Sample Typ:e Cell cultures (adherent and suspension), parafin embedded tissue sections

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Features and Benefits:

• Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive and stable • The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.