Description
Alpha 1-Antitrypsin (Rat) ELISA Kitis available at Gentaur for Next week Delivery.
A Sandwich ELISA kit for the quantitative measurement of rat Alpha 1-Antitrypsin in serum, plasma and other biological fluids..
Description:
Alpha 1-Antitrypsin is an inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin, and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. BioVision’s Alpha 1-Antitrypsin (Rat) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Alpha 1-Antitrypsin. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Rat Alpha 1-Antitrypsin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of Alpha 1-Antitrypsin bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat Alpha 1-Antitrypsin. The concentration of Rat Alpha 1-Antitrypsinin the samples can be calculated by comparing the OD of the samples to the standard curve.
Applications: In vitro quantitative determination of rat Alpha 1-Antitrypsin concentrations in serum, plasma and other biological fluids
Sample Typ:e Plasma,Serum,Other biological fluids
Alternate Name: SERPINA1, A1A, A1AT, AAT, PI, PI1, PRO2275
Features and Benefits:
Detection Range: 6.25-400 ng/mL,Sensitivity: 3.75 ng/mL,Precision: Coefficient of variation is < 10%,Specificity: No Significant cross-reactivity or interference between Rat α1-AT and analogues was observed.
Additional Information
Size: |
96 assays |
Storage Conditions: |
4°C4ºC |
Shipping Conditions: |
Gel Pack |
Shelf life: |
6 months |
Detection Method: |
Sandwich ELISA, Absorbance (450 nm) |
Category: |
ELISA |