Acid Sphingomyelinase Assay Kit II (Colorimetric)

Acid Sphingomyelinase Assay Kit II (Colorimetric)is available at Gentaur for Next week Delivery.

Complete kit that allows the measurement of Acid Sphingomyelinase in biological samples.

Description:

Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyelinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.

Applications: Measurement of ASMase activity in various tissues/cell extracts

Sample Typ:e Cell lysate,Serum,Tissue lysate

Alternate Name: Acid Sphingomyelinase,SMase,Sphingomyelinase

Features and Benefits:

Complete set of reagents for the detection of Acid SMase in biological samples,Simple, specific (detect acid SMase activity only)