Phospho-C-RAF (Ser301) Antibody

Phospho-C-RAF (Ser301) Antibodyis available at Gentaur for Next week Delivery.

Description: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity.

Immunogen Sequence:A synthesized peptide derived from human C-RAF around the phosphorylation site of Sersine 301.

Target Alternative Name:RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF

Species Reactivities:Rat, Mouse, Human

Tag Line:Polyclonal antibody to detect endogenous levels of Phospho-C-RAF (Ser301)

Formulation:In phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol

Purification: Affinity purified

Application:Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blotting, ELISA

Positive Control:/

Application And Usages:WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA (peptide) 1:20000-1:40000

Handling:The antibody solution should be gently mixed before use.