Description
Thioredoxin Reductase 1 Antibody (19A1)is available at Gentaur for Next week Delivery.
Description: The mammalian thioredoxin reductases (TrxRs) are a family of seleno-cysteine containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate seleno-cysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55 – 58 kDa in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena. TrxR1 plays a central role as a glucosyl donor in cellular metabolic pathways.
Immunogen Sequence:Recombinant human protein purified from E.coli
Target Alternative Name:Thioredoxin Reductase, GRIM-12, mgC9145, TR, TR1, TRXR1, TXNR.
Species Reactivities:Human
Tag Line:Monoclonal Antibody to detect thioredoxin reductase I in human samples.
Formulation:100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Purification: Ammonium sulphate precipitation purified antibody
Application:Western blot, ELISA.
Positive Control:WB analysis of HeLa and Jurkat cell lysates.
Application And Usages:Western blot: 1:1000, IP: 1-2 µl, ELISA.
Handling:The antibody solution should be gently mixed before use.
Additional Information
Size: |
100 μl |
Antibody Target: |
Thioredoxin Reductase 1 |
Category: |
Signal Transduction |
Lot Number: |
80764 |
Host: |
Mouse |
Antibody Type: |
Monoclonal |
Isotype: |
Mouse IgG 1 |
Accession Number: |
Q16881 |
Gene Id: |
7296 |
Concentration: |
1 mg/ml |
Appearance: |
Colorless liquid |
Form: |
Liquid |
Storage Condition: |
-20 °C |
Shipping Condition: |
gel pack |
Shelf Life: |
12 months |
Country of Animal Origin: |
USA |
Country of Manufacture: |
USA |
Usage: |
For Research Use Only! Not to be used in humans. |
Western Blot Verified: |
Verified |
Immunocytochemistry Verified: |
Not Verified |
Immunofluorescence Verified: |
Not Verified |
Immunoprecipitation Verified: |
Not Verified |
ELISA Verified: |
Not Verified |
ChIP Verified: |
Not Verified |
Flow Cytometry Verified: |
Not Verified |