PUMA/bbc3 Antibody

PUMA/bbc3 Antibodyis available at Gentaur for Next week Delivery.

Description: PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Immunogen Sequence:Synthetic peptide surrounding amino acid 177 of human PUMA

Target Alternative Name:PUMA/JFY1 ,JFY-1, PUMA, BBC3, bcl-2 binding component 3

Species Reactivities:Human, mouse, rat

Tag Line:/

Formulation:30 µg (0.5 mg/ml) affinity purified rabbit anti-PUMA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Application:Western blot, Immunohistochemistry

Positive Control:Western Blot: Jurkat cells and Rat kidney tissue, IHC: Tonsil tissue

Application And Usages:Western blotting (0.5-4 µg/ml)and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.

Handling:The antibody solution should be gently mixed before use.