Phospho-FoxO4/AFX Antibody

Phospho-FoxO4/AFX Antibodyis available at Gentaur for Next week Delivery.

Description: The forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX, AND FKHRL1) have sequence similarity to the nematode homolog Daf 16, recently shown to mediate signaling via a pathway involving IGFR1, P13-K and Akt. There are three potential Akt phosphorylation sites on the FKHR proteins. One site, FKHR Ser256 is found in the Forkhead domain and the other two are outside this domain. Recent studies show that phosphorylation of FKHR family members by Akt promotes cell survival. Phosphorylation regulates FKHR nuclear translocation and target gene transcription. Insulin also stimulates FKHR phosphorylation at Ser253.

Immunogen Sequence:Synthetic peptide corresponding to residues surrounding Ser193 of human FoxO4/AFX

Target Alternative Name:AFX1, MLLT7, Forkhead box protein O4; Fork head domain transcription factor AFX1

Species Reactivities:Human, mouse

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Formulation:100 µg (0.5 mg/ml) peptide affinity purified polyclonal rabbit anti-Phospho-AFX antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide

Purification: Affinity purified

Application:Western blot

Positive Control:/

Application And Usages:Western blotting (1-2 µg/ml). However, the optimal concentrations should be determined individually. Phospho-AFX (Ser193) antibody detects only the phosphorylated Ser193 of AFX and does not react with nonphosphorylated AFX.

Handling:The antibody solution should be gently mixed before use.