Nitrotyrosine Antibody (NOY-7A5)

(No reviews yet) Write a Review
SKU:
5415-50
€415
Frequently bought together:

Description

Nitrotyrosine Antibody (NOY-7A5)is available at Gentaur for Next week Delivery.

Description: Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is a marker for inflammation and nitric oxide (NO) production. It is detected in large number of pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosine residues important for signal transduction. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, chronic renal failure and keratoconus. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples.

Immunogen Sequence:KLH (keyhole limpet hemocyanin) modified with nitrotyrosine.

Target Alternative Name:/

Species Reactivities:All species

Tag Line:/

Formulation:In 1 ml 2x PBS, 0.09% sodium azide, PEG and sucrose.

Purification: /

Application:Western Blot

Positive Control:/

Application And Usages:Western Blot (use 1 µg/ml for AP/BCIP/NBT (MTT) detection and 0.5 µg/ml for HRP/ECL detection). Recommended blocking buffer is CPPT: 10 mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, and 150 mM sodium chloride.

Handling:The antibody solution should be gently mixed before use.

View AllClose

Additional Information

Size:
50 µg
Antibody Target:
Nitrotyrosine
Category:
Nitrotyrosine
Lot Number:
70915
Host:
Mouse
Antibody Type:
Monoclonal
Isotype:
Mouse IgG1
Appearance:
Colorless liquid
Form:
Liquid
Storage Condition:
-20 °C
Shipping Condition:
gel pack
Shelf Life:
12 months
Country of Animal Origin:
USA
Country of Manufacture:
USA
Usage:
For Research Use Only! Not to be used in humans.
Western Blot Verified:
Not Verified
Immunocytochemistry Verified:
Not Verified
Immunofluorescence Verified:
Not Verified
Immunoprecipitation Verified:
Not Verified
FACS Verified:
Not Verified
ELISA Verified:
Not Verified
ChIP Verified:
Not Verified
Flow Cytometry Verified:
Not Verified
View AllClose

0 Reviews

View AllClose