H3K27me3S28p polyclonal antibody

H3K27me3S28p polyclonal antibodyis available at Gentaur for Next week Delivery.

Description: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Phosphorylation of H3 on serine 28 is increased during mitosis.

Immunogen Sequence:KLH-conjugated synthetic peptide of Histone H3 containing trimethylated lysine 27 and the phosphorylated serine 28.

Target Alternative Name:Histone H3

Species Reactivities:Human.

Tag Line:Polyclonal Antibody to detect H3K27me3S28p in human samples.

Formulation:In PBS with 0.05% (W/V) sodium azide.

Purification: Whole antiserum from rabbit

Application:Western blot, IF, ELISA, ChIP, Dot blot.

Positive Control:Western blot: HeLa cells, IF: HeLa cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides.

Application And Usages:IF: 1:200, WB: 1:250 – 1:500, ELISA: 1:100 – 1:500, Dot Blot: 1:20,000, ChIP: 1 µl/ChIP, IP: 5 µl/IP.

Handling:The antibody solution should be gently mixed before use.