H3K27me3 polyclonal antibody

H3K27me3 polyclonal antibodyis available at Gentaur for Next week Delivery.

Description: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K27 is associated with gene repression.

Immunogen Sequence:Polyclonal antibody raised in rabbit against histone H3, trimethylated at lysine 27 (H3K27me3), using a KLH-conjugated synthetic peptide.

Target Alternative Name:Histone 3

Species Reactivities:Human, mouse.

Tag Line:Polyclonal Antibody to detect H3K27me3 in human and mouse samples.

Formulation:In PBS with 0.05% sodium azide and 0.05% ProClin 300.

Purification: Affinity Purified

Application:Western blot, IF, ELISA, Dot Blot, ChIP.

Positive Control:Western blot: Whole cell and histone extracts, IF: NIH3T3 cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides.

Application And Usages:WB: 1:500, Dot Blot: 1:5,000, ChIP: 1 µl/ChIP, IF: 1:200, ELISA: 1:200.

Handling:The antibody solution should be gently mixed before use.