Dnmt2 Antibody

Dnmt2 Antibodyis available at Gentaur for Next week Delivery.

Description: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Immunogen Sequence:Synthetic peptides surrounding amino acid 42 and 364 of mouse Dnmt2

Target Alternative Name:Dnmt2 , TRDMT1 , RNMT1 , OTTHUMP00000045198 , cytosine-5-methyltransferase , EC 2.1.1.29

Species Reactivities:Human, mouse

Tag Line:/

Formulation:100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Application:Western blot, Immunofluorescence

Positive Control:/

Application And Usages:Western blotting (0.5-4 µg/ml) and immunofluorescence. However, the optimal concentrations should be determined individually. The antibody recognizes 42 kDa Dnmt 2 of human and mouse origins. Mouse testes tissue lysate can be use as a positive control.

Handling:The antibody solution should be gently mixed before use.