D-Tag Antibody

D-Tag Antibodyis available at Gentaur for Next week Delivery.

Description: DYKDDDDK Tag antibody detects over-expressed proteins containing the D epitope tag fused to either the amino- or carboxy-terminus of targeted proteins. Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The DYKDDDK epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems

Immunogen Sequence:A synthetic D- tag peptide DYKDDDDK conjμgated to KLH.

Target Alternative Name:D-Tag, anti-D-Tag, D-Tag antibody,D-Tag antibody

Species Reactivities:All species

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Formulation:30 µg (0.5 mg/ml) affinity purified rabbit anti-D-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.01 % thimerosal.

Purification: Affinity purified

Application:Western blot

Positive Control:MYC/D- tagged protein ladder (Lane 1)

Application And Usages:The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.

Handling:The antibody solution should be gently mixed before use.