Anti-HIF-1-alpha, Rabbit Monoclonal Antibody

Anti-HIF-1-alpha, Rabbit Monoclonal Antibodyis available at Gentaur for Next week Delivery.

Description: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

Immunogen Sequence:A peptide corresponding to Hypoxia-inducible factor 1-alpha

Target Alternative Name:ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Hypoxia-inducible factor 1-alpha, bHLHe78, Member of PAS protein 1

Species Reactivities:Human

Tag Line:Rabbit monoclonal antibody to detect HIF-1-alpha in human samples.

Formulation:In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Purification: Affinity purified using protein A

Application:IHC, WB

Positive Control:WB: Jurkat cell lystae; IHC: human breast cancer tissue

Application And Usages:IHC: 1:500 -1:1000 dilution; WB: 1:1000 - 1:2000 dilution.

Handling:The antibody solution should be gently mixed before use.