Anti-Cyclin D1, Rabbit Monoclonal Antibody

Anti-Cyclin D1, Rabbit Monoclonal Antibodyis available at Gentaur for Next week Delivery.

Description: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.

Immunogen Sequence:A peptide corresponding to Cyclin D1

Target Alternative Name:G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, PRAD1

Species Reactivities:Human

Tag Line:Rabbit monoclonal antibody to detect Cyclin D1 in human samples.

Formulation:In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Purification: Affinity purified using protein A

Application:IHC, WB

Positive Control:WB: Hela cell lysate; IHC: human breast cancer tissue

Application And Usages:IHC: 1:500 -1:1000 dilution; WB: 1:1000 - 1:2000 dilution.

Handling:The antibody solution should be gently mixed before use.