5-hmC polyclonal antibody (rabbit)

5-hmC polyclonal antibody (rabbit)is available at Gentaur for Next week Delivery.

Description: 5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0.6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is abundant in brain tissue, especially in areas that are associated with higher cognitive functions. Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. The results shown here illustrate the use of this unique rabbit polyclonal antibody against 5-hydroxymethylcytosine that has been fully validated in various technologies.

Immunogen Sequence:Polyclonal antibody raised in rabbit against 5-hydroxymethylcytosine conjugated to KLH.

Target Alternative Name:5-hydroxymethylcytosine

Species Reactivities:Human, mouse, wide range.

Tag Line:Polyclonal Antibody to detect 5-hmC in human samples.

Formulation:In PBS with 0.05% (W/V) sodium azide.

Purification: Whole Anti-Sera

Application:ELISA, hmEdIP, Dot blot.

Positive Control:ELISA: Peptide, hmEdIP, Dot blot: Peptides.

Application And Usages:hMeDIP: 2.5 µg/IP, Dot Blot: 1:200, ELISA: 1:500.

Handling:The antibody solution should be gently mixed before use.